Biocompatibility of Two Novel Root Repair Materials

Abstract

Introduction

Jingzhi Ma, DDS, MSD, Ya Shen, DDS, PhD, Sonja Stojicic, DDS, MSD, Markus Haapasalo, Dr Odont

published online 14 April 2011.

The purpose of the present study was to evaluate the biocompatibility of 2 root-end filling materials, Endosequence Root Repair Material Putty (ERRM Putty) and Paste (ERRM Paste) and compare them with gray mineral trioxide aggregate (MTA).

Methods: ERRM Putty, ERRM Paste, MTA, intermediate restorative material (IRM), and Cavit G were tested. For cytotoxicity assay, human gingival fibroblasts were incubated for 1, 3, and 7 days with extracts of varying concentrations from materials set for 2 days or 7 days. Cell viability was evaluated by methyl-thiazol-tetrazolium (MTT) assay. For cell adhesion assay, materials set for 7 days were examined under scanning electron microscope directly after setting, after incubation in cell culture medium for 7 days, and after incubation in gingival fibroblast suspension at a density of 5 × 104 cells/well for 2 and 7 days. The constituents of crystals formed on surface of materials were determined by energy dispersive analysis by x-ray.

Results: Cell viability was significantly correlated with the type of material, setting time, and incubation time (P < .001 for all parameters). ERRM Putty and ERRM Paste displayed similar cell viabilities to MTA at all experimental conditions, except that fresh samples of ERRM Paste showed slightly lower cell viabilities than MTA. Cell viabilities with IRM and Cavit G were significantly lower than with the other 3 materials (P < .001). Similar surface crystallographic features and cell adhesion were observed on ERRM Paste, ERRM Putty, and MTA.

Conclusions: ERRM Putty and ERRM Paste displayed similar in vitro biocompatibility to MTA.

CLINICAL CASE:

Restorative treatment plan was a crown on 37. I had a hard time removing the silver points and extruded gutta-percha. One silver point fractured in the apical third, I couldn’t retrieve it, so I decided to leave it alone. Eventually I could remove the extruded gutta-percha, with a thin explorer that I adjusted a bit, by bending the tip into a sharp curve. In this case, I used Brasseler MTA putty. Initially, I was not thrilled about its handling properties, but I am starting to like it, in particular when there is quite a bit of moisture, the consistency is less sandy than MTA, which makes it stay in place better, it doesn’t wash out that easily. Composite build-up and 2 posts.

Marga Ree