Oral Health Group

Clinical Report

March 1, 2010
by Dr Adam Alford, BDS (Hons)


‘At-Home Vital Tooth Bleaching’ (AHVTB) or ‘Night Guard Vital Bleaching (NGVB)’ has become one of the most asked for and practiced aesthetic treatment available to dentists. At home tooth whitening produces some of the most satisfying results for the patient and dentist whilst also being the most conservative form of aesthetic dentistry. In today’s society where personal appearance has become very much associated with self-confidence, being able to achieve a nicer whiter smile for patients with minimal intervention can also help to build a patience self confidence and self-esteem.37,12

“As early as 1877 dental researcher discovered oxalic acid could be used to whiten vital teeth”.37 “In the early twentieth century, the use of 35% hydrogen peroxide was recognised as the most effective bleaching agent. In 1950, Pearson administered heat and hydrogen peroxide for non-vital teeth bleaching. In 1976, Nutting and Poe introduced the walking bleach technique, which uses 35% hydrogen peroxide and sodium perborate for non-vital teeth bleaching (Sun, 2000).”


“For Vital teeth bleaching, in 1918, Abbot used high-intensity light, raising the temperature of the hydrogen peroxide rapidly to accelerate the chemical process of bleaching. In the late 1960’s, a successful technique for home bleaching was introduced by Klusmier, at which time he discovered that 10% carbamide peroxide was loaded in a mouth guard with the intent to improve the gingival condition also resulted in a bleaching effect. By March 1989, Haywood and Heymann introduced and published this technique; in the 1990’s, this procedure has been used widely by the dental community (Sun, 2000).”

The whitening effect is due to the high molecular weight hydrogen peroxide decomposing rapidly into various free radical ions. These ions react with the long-chained, dark-coloured chromophile molecules, breaking into smaller, lighter coloured structures. It also could be the phenomenon of altering the optical structure of the chromophile molecule, rendering the stain invisible. Both the dentine and enamel change colour due to the easy passage of the hydrogen peroxide through the tooth structure.44, 12,40

‘At -Home Vital Tooth Bleaching’ (AHVTB) has been studied and used for many years now and has been found to be safe and effective in whitening patients teeth. It has also been shown that AHVTB does not have any detrimental long term effects on the teeth.36,4,25,48

The main transient side effects are tooth sensitivity (TS) and gingival irritation (GI), gastric irritation and sore throat have also been noted at times. The side effects on average take 2-7 days to subside, most side effects though subside before the completion of the treatment.1-4,8-10,12,16-19,24-28, 30-34,36,37,43,46,47 Sensitivity is in the form of a reversible pulpitis caused from the dentinal fluid flow and pulpal contact of the material without apparent harm to the pulp16. The degree of sensitivity ranges from mostly mild to moderate with occasional severe sensitivity.

“Double-blind clinical studies have shown that sensitivity occurs in 55%-75% of treatment groups. The placebo groups also experienced between 20% and 30% sensitivity. One study even reported tooth sensitivity of about 15% in subjects wearing only the bleaching tray. Therefore it appears that sensitivity is a multifactorial event that cannot be totally avoided, because it is not exclusively related to the peroxide whitening material (Haywood, 2001).”

The side effects of gingival irritation (GI) and tooth sensitivity (TS) are the most common side effects and affect the patients’ compliance to carry out the full course of treatment. Other issues with compliance are the number of applications required to maximise the change of colour. It has been shown that the teeth whiten to a point rapidly and then plateau and it is difficult to whiten past this point.12 Indicating an ideal ‘end point’ (Smart, 2004, comment) for bleaching.

Compliance is the main problem with AHVTB, there have been numerous studies on increasing the concentration of materials so as to decrease the number of applications necessary to reach a satisfactory outcome. Many articles have also looked at using desensitising agents such as potassium nitrate and fluoride to reduce tooth sensitivity therefore increasing compliance.46 The desensitizing agents can be included in the bleaching material or applied separately before or after treatment.

“Tooth sensitivity has been attributed to the penetration of hydrogen peroxide into the pulp chamber. It is speculated that reducing the hydrogen peroxide concentration or the duration of bleach application could reduce the tooth sensitivity but would also likely reduce the tooth whitening as well. Hence other desensitising agents, such as potassium nitrate, have been added to carbamide peroxide bleach formulations in an attempt to decrease the tooth sensitivity experienced by the patient without reducing the concentration of the active bleaching ingredient. (Tam, 2001)”

This study was designed to produce a protocol for the general practitioner that combines known treatment methods and concentrations of materials with known trends, so as to try and decrease the number of applications necessary by using higher concentrations of materials in an incremental approach, also combining the use of active and passive treatment modalities for side effects, so that the main side effects were kept at tolerable levels for the patient within normal ranges of experienced side effects form other literature. The patients were instructed on what to expect and how to treat their own symptoms with the materials given to them, so as to try and improve the outcome by giving the patient enough understanding to control their situation.

Materials and Methods


The materials tested were 3, 7.5 and 9.5% hydrogen peroxide (Pola Day by SDI) and 10, 16 and 22% carbamide peroxide (Pola Night by SDI);


Pola Day Pola Night

3-9.5% Hydrogen peroxide (HP) 10-22.0% wt Carbamide peroxide (CP)

<47% wt Additives <40% wt Additives

30% wt Glycerol 30% wt Glycerol

0.1%wt Flavour 0.1% wt Flavour

Neutral pH Neutral pH

20% Water 20% Water

The desensitizing agents used were 6% Potassium Nitrate and 0.10% Sodium Fluoride material in combination (Soothe, SDI) and 3.75% Potassium Chloride and 0.31% Sodium Fluoride (Sensodyne toothpaste, GSK)

Soothe (SDI) Sensodyne Toothpaste

Potassium Nitrate 6.0% Potassium Chloride 3.75%

Fluoride ions 0.10% Sodium Fluoride 0.31% (1400ppm)

Water 89.60% Triclosan 0.3%

Thickener 4.20%

Sodium benzoate 0.10%


The study population were patients from private practice who had expressed an interest in whitening their teeth. Patients that had indicated on medical history forms that they had acatalasaemia or glucose-6-phosphate dehydrogenase deficiency (G6PD), were not included in the study population. The ages ranged from 25-65. There were 30 patients surveyed, of which 19 filled in their questionnaires properly.

Impressions were taken on patients and soft bleaching trays were made by an independent lab. The lab was instructed to place reservoirs on all teeth and also not to trim the trays to the gingival margin but to leave the trays approximately 4-5 mm above the gingival margin, so the trays covered the gums and had enough clearance material so as not to press into the gum while seated.

The patients were instructed on the use of the materials ad given an instruction sheet (Refer to Appendix One). The instructions verbally given to the patients were to apply a small amount of the material into the tra
ys of approximately a quarter a pea size amount. Place this amount in each tooth space on the front surface of the trays. The patients were instructed that if they were using half to three-quarters of the tube for both upper and lower trays they were using approximately the right amount of material. The patients were to seat the trays and wipe away excess material and then wear the trays for approximately 2 hours, then remove the trays rinse mouth and trays out, and then place one of the desensitising agents into the trays for another half an hour. Then remove the trays wait at least another half and hour then clean the teeth.

The patients were split into two groups one used hydrogen peroxide 3%, 7.5% and 9.5% materials (Pola Day, SDI), the other group used 10%, 16% and 22% carbamide peroxide materials (Pola Night, SDI).

Order of bleaching materials hydrogen peroxide

1. For the first 4 applications of the whitening agent, please use the 3% whitening material and Sensodyne toothpaste.

2. For the next 6 applications please use the 7.5% whitening material and Soothe or Sensodyne depending on how sore or sensitive your teeth are. The Soothe material is a stronger desensitiser.

3. If you require a lighter colour the next 4 applications can be made with the 9.5% whitening material with the use of Soothe.

Order of bleaching materials carbamide peroxide

1. For the first 4 applications of the whitening agent, please use the 10% whitening material and Sensodyne toothpaste

2. For the next 6 applications please use the 16% whitening material and Soothe or Sensodyne depending on how sore or sensitive your teeth are. The Soothe material is a stronger desensitiser.

3. If you require a lighter colour the next 4 applications can be made with the 22% whitening material with the use of Soothe.

The patients were also instructed that they would most likely experience some tooth sensitivity and gum irritation and that if it is only mild then they should use the Sensodyne toothpaste in the trays after whitening for half an hour, if the tooth sensitivity or gum soreness was of a moderate level they could choose between the Soothe or Sensodyne toothpaste to use within the trays for half an hour after whitening. The patients were instructed that if the tooth sensitivity or gum soreness was greater than moderate and becoming uncomfortable then they should take a days break and/or use the Soothe material without whitening for 2 hours in the trays. It was mentioned to the patients that it was unnecessary to bleach everyday, but that it was preferred that they whiten for a few days and take a days rest then whiten again for a few days and then rest. The patients were also instructed that if the sensitivity became severe then they were to stop bleaching and contact the researcher. It was explained to the patients they were in control of their symptoms and instructed how to use the materials when necessary or take time off from the whitening procedure, therefore hopefully making the patient more comfortable with the procedure and more compliant.

The patients were given questionnaires to fill one out everyday, these questionnaires involved yes/no questions and three visual analogue scales (VAS) of 10cm’s with 10 increments form 0-10, for the patients to rate their tooth sensitivity, gum soreness and overall discomfort levels on 46. The patients were also asked if they would like to make any other comments as well. (Refer to Appendix One)

The patients were reviewed at the approximately 7 days and once the patient was satisfied with the result, or if the patient was experiencing above normal tooth sensitivity or gum soreness.


The Patients were instructed to fill out a questionnaire with a series of yes / no answers and three Visual Analogue Scales (VAS)46. Results are presented in the following graphs and tables.


The study was designed to try and combine previously recognised trends in the literature in a clinical protocol that the general practitioner can follow so as to try and improve patient compliance by reducing the number of applications of the material needed to reach a satisfactory end point. The study combined the use of desensitising materials and known methods for reducing common side effects, of tooth sensitivity (TS) and gingival irritation (GI), with the incremental increase in concentration of the peroxide materials so as to try and limit the number of applications of bleaching materials necessary whilst maintaining the side effects, reported in previous studies, within normal intensity and frequency gradients. The study also tried to enable the patients to make informed decisions about how to treat their own symptoms, this is an at home monitored system where the patient is in complete control of the treatment outcome, so by hopefully improving the patients awareness of available treatment protocols for the man side effects and giving the patients access to the necessary treatment options for the main side effects, it was hoped that this would improve patient compliance and the overall treatment outcome.

The patients were private practice patients that expressed a wish to whiten their teeth. This was due to the fact the study was trying to generate information to guide the private practitioner in the normal general practice environment. Unfortunately this also meant that at times it was hard to get patients to fill in their forms properly and compliance has even more of a negative impact on the outcomes, but it also meant that patients were more expectant of results and less willing to endure the main side effects if they occurred, so therefore more willing to use the treatment methods available to them whilst being more honest with filling in the questionnaires if they were completed.


The trend indicates that by incrementally increasing the concentrations of materials and by educating the patients to the active and passive treatment modalities available to the patient for the main side effects, of tooth sensitivity and gingival irritation, that an acceptable result can be achieved for both patient and practitioner in the least number of applications necessary whilst maintaining the main side effects within or below the normal levels achieved in other literature. Therefore, improving patient compliance and the overall satisfaction level of the process.

Special Thanks

To SDI for donating the materials necessary for the trial, and to Roseneath Dental Surgery, London for supplying the practice environment necessary for the trial, and to the patients of the Roseneath Dental Surgery, London involved in the trial.


1. Albuquerque, Gomez, Dutra, Vasconcellos, Effects of a 10% Carbamide Peroxide Bleaching Agent on Rat Oral Epithelium Proliferation, Brazil Dental Journal, Volume 13(3), 162-165, 2002,

2. Bizhang et al, Intra-coronal Bleaching of Discoloured Non-Vital Teeth, Operative Dentistry, 2003, 28-4, 334-340

3. Boyd, Treatment of Discoloured Teeth, Health and Law Magazine, June 1997

4. Collins et al, Safety evaluation of a novel whitening gel, containing 6% hydrogen peroxide and a commercially available whitening gel containing 18% carbamide peroxide in an exaggerated use clinical study, Journal of Dentistry, Vol. 32, 2004, 47-50

5. Donly, The Adolescent Patient: Special Whitening Challenges, Compendium, Vol 24, No. 4A, Apirl 2003

6. Feildhouse, Teeth Whitening Debate I (letter), British Dental Journal, Vol. 193. No.6, September 28 2002, 300

7. Gerlach, Shifting Paradigms in Whitening: Introduction of a Novel System for Vital Tooth Bleaching, Compendium, Volume 21(29), S4-S9, 2000

8. Gerlach, Vital Bleaching With Whitening Strips: Summary of Clinical Research on E
ffectiveness and Tolerability, The Journal of Contemporary Dental Practice, Volume 2 (3), Summer Issue, 1-15, 2001

9. Gerlach, Sagel, Jeffers, Zhou, Effect of Peroxide Concentration and Brushing on Whitening Clinical Response, Compendium, Volume 23(1A), 16-21, 2002

10. Gerlach, Zhou, Comparative Clinical Efficacy of Two Professional Bleaching Systems, Compendium, Volume 23 (1A), 35-41, 2002

11. Gerlach et al, Removal of Extrinsic Stain Using a 7.0% Sodium Hexametaphosphate Dentrifice: A Randomized Clinical Trial, The Journal of Clinical Dentistry, Vol. XIII, No.1, 6-9

12. Haywood, Current Status of Nightguard Vital Bleaching, Compendium, Volume 21(28), June 2000, S10-S17, June 2000

13. Haywood, Dentine hypersensitivity: bleaching and restorative considerations for successful management, International Dental Journal, Vol. 52, 2002, 376-384

14. Haywood, Frequently Asked Questions About Bleaching, Restorative and Aesthetic Practice, Vol. 6, No. 1, Spring 2004, 22- 34

15. Haywood, Quick Tips Nightguard Vital Bleaching and In-Office Bleaching, Contemporary Esthetics and Restorative Practice, July/August, 78-81, 1998,

16. Haywood, Caughman, Frazier, Tray delivery of potassium nitrate-fluoride to reduce bleaching sensitivity, Quintessence International, Volume 32 (2), 105-108, and 2001

17. Heymann et al, Clinical Evaluation of Two Carbamide Peroxide Tooth-Whitening Agents, Compendium, Vol 19, No 4, April 1998

18. Jacobsen, Bruce, Clinical Dentin Hypersensitivity: Understanding the Causes and Prescribing a Treatment, The Journal of Contemporary Dental Practice, Volume 2(1), Winter Issue, 1-8, 2001

19. Jorgensen, Carroll, Incidence of Tooth Sensitivity after home whitening treatment, JADA, Vol 133, August 2002

20. Jorgensen et al, Correction ‘Incidence of Tooth Sensitivity After Home Whitening Treatment”, JADA, Vol. 133, September 2002, 1174

21. Kashima-Tanaka et al, Generation of Free Radicals and/or Active Oxygen by Light or Lazer Irradiation of Hydrogen Peroxide or Sodium Hypochlorite, Journal of Endodontics, Vol 29, No. 2, February 2003.

22. Kihn et al, A Clincal Evaluation of 10% VS 15% Carbamide Peroxide Tooth-Whitening Agents, JADA, Vol 131, October 2000, 1478-1484

23. Kugel, Aboushala, Zhou, Gerlach, Daily use of Whitening Strips on Tetracycline-Stained Teeth: Comparative Results After 2 Months, Compendium Volume 23 (1A), 29-34, 2002

24. Leonard, Garland, Eagle, Caplan, Safety Issues When Using a 16% Carbamide Peroxide Whitening Solution, Journal of Esthetic and Restorative Dentistry 14:358-367, 2002

25. Li, Tooth Bleaching Using Peroxide-Containing agents: Current Status of Safety issues, Compendium, Vol19, No. 8, August 1998, 783-795

26. Matis, Jiang, Eckert, Extended at-home bleaching of tetracycline-stained teeth with different concentrations of carbamide peroxide, Quintessence International, Volume 33 (9), 645- 655, 2002

27. Matis et al, The efficacy and safety of a 10% carbamide peroxide bleaching gel, Quintessence international, Volume 29, Number 9, 1998, 555-563

28. Mokhlis et al, A Clinical Evaluation of Carbamide Peroxide and Hydrogen Peroxide Whitening Agents During Daytime Use, JADA, Vol. 131, September 2000, 1269-1277

29. Moreirs de Freitas, Basting, Rodrigues, Serra, Effect of two 10% peroxide carbamide bleaching agents on dentin microhardness at different time intervals, Quintessence International Volume 33(5), 370-374

30. Nathoo et al, Comparative Seven-Day Clinical Evaluation of Two Tooth Whitening Products, Compendium Vol.22, No. 7, July 2001

31. Nathoo et al, Comparative clinical Investigation of the Tooth Whitening Efficacy of Two Tooth Whitening Gels, J Clin Dent, Vol. XIV, No. 3, 2003, 64-69

32. Niederman et al, Effectiveness of Dentist-Prescribed, Home-Applied Tooth Whitening, A Meta-analysis, The Journal of Contemporary Dental Practice, Volume 1, No. 4, Fall Issue, 2000

33. Niessen Talking with Patients; Tooth Whitening, Why, Who, Where, What, and How, , Journal of Esthetic and Restorative Dentistry, Volume 13, Number 1, 2001

34. Pohjola et al, Sensitivity and Tooth Whitening Agents, J Esthet Restor Dent 14;85-91,2002, Abstract

35. Price et al, The pH of Tooth-Whitening Products, Journal of Canadian Dental Association, Vol. 66, No.8, September 2000, 421-426

36. Ritter et al, Saftey and Stability of Nightguard Vital Bleaching: 9-12 Years Post-treatment, Esthet Restor Dent 14:275-285, 2002

37. Sagel, Odioso, McMillan, Gerlach, Vital Tooth Whitening With a Novel Hydrogen Peroxide Strip System: Design, Kinetics, and Clinical Response, Compendium, Volume 21(29), S10-S15, 2000

38. Sarrett, Tooth Whitening Today, JADA Vol. 133, November 2002, 1535-1538

39. Saxer, Yankell, Impact of improved toothbrushes on dental diseases. II, Quintessence International, Vol. 28, No.9, 1997, 573-593

40. Scientific Committee on Cosmetic Products and Non-Food Products (SCCNFP), Opinion of the Scientific Committee on Cosmetic Products and Non-Food Products Intended for Consumers Concerning Hydrogen (Carbamide, Zinc) Peroxide in Tooth Bleaching/ Whitening Products, 21st plenary meeting of 17th September 2002.

41. Seward, Chief Dental Officer, Teeth whitening debate II (comment), British Dental Journal, Volume 193. No. 6, September, 2002

42. Stewart et al, Comparative Clinical Investigation of the Tooth Whitening Efficacy of Two Tooth Whitening Gels, J Clin Dent 14:64-69, 2003

43. Sulieman, Addy, MacDonald, Rees, The effect of hydrogen peroxide concentration on the outcome of tooth whitening: an in vitro study, Journal of Dentistry 32, 2004, 295-299

44. Sun, The role of lazers in cosmetic dentistry, Dental Clinics of North America, Volume 44, number 4, October 2000, 831-850

45. Tam, Clinical Trial of Three 10% Carbamide Peroxide Bleaching Products, Canadian Dental Association Journal, Volume 65 (4), 201-206, April 1999,

46. Tam, Effect of potassium nitrate and fluoride on carbamide peroxide bleaching, Quintessence International, Volume 32 (10), 766-770, 2001

47. Tsubura, Yamaguchi, Clinical Evaluation of New Bleaching Product “Pola Night” on Japanese population, The Nippon Dental University School of Dentistry

48. Ulukapi, Benderli, Ulukapi, Effect of pre- and postoperative bleaching on marginal leakage of amalgam and composite restorations, Quintessence International Volume 34(7), 505-508, 2003

49. Walsh, The Current Status of Laser Applications in Dentistry, Australian Dental Journal 2003; 48(3):146-155

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